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Monoclonal antibodies recognizing the Enterococcus faecalis collagen-binding MSCRAMM Ace: conditional expression and
Andrea E Hall1, Elena L Gorovits, Peter J Syribeys
1Inhibitex, Inc., 9005 Westside Parkway, Alpharetta, GA 30004, USA.
Microbial Pathogenesis
|May 25, 2007
Summary
Enterococcus faecalis uses the Ace adhesin to bind host tissues, aiding infection. Researchers developed monoclonal antibodies against Ace, potentially useful for studying bacterial adhesion and treating infections.
Area of Science:
- Microbiology
- Immunology
- Pathogenesis
Background:
- Enterococci are opportunistic pathogens causing human infections.
- Bacterial adhesion to host extracellular matrix (ECM) is key in pathogenesis.
- The Enterococcus faecalis adhesin of collagen (Ace) facilitates ECM binding.
Purpose of the Study:
- To characterize Ace expression and binding to host cells and ECM.
- To generate and characterize monoclonal antibodies (mAbs) against the Ace adhesin.
- To explore the therapeutic potential of anti-Ace mAbs.
Main Methods:
- In vitro assays to study Ace expression and binding.
- Surface plasmon resonance and flow cytometry for mAb characterization.
- Generation of a panel of monoclonal antibodies against Ace.
Main Results:
- Ace binds to mammalian epithelial and endothelial cells.
- Ace demonstrates binding to ECM collagens.
- A panel of functional anti-Ace mAbs was successfully generated and characterized.
Conclusions:
- Ace is crucial for Enterococcus faecalis adhesion to host tissues and ECM.
- Characterized mAbs can aid research into E. faecalis adhesion mechanisms.
- Anti-Ace mAbs show promise as therapeutic agents against E. faecalis infections.
