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Updated: Jul 14, 2026

In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Trigeminal Ganglia

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Sodium currents in mesencephalic trigeminal neurons from Nav1.6 null mice.

Akifumi Enomoto¹, Juliette M Han, Chie-Fang Hsiao

¹Department of Physiological Science, University of California, Los Angeles, California 90095, USA.

Journal of Neurophysiology

|May 25, 2007

Summary

Subthreshold sodium currents, particularly resurgent currents, are crucial for rhythmic bursting in mesencephalic trigeminal neurons. Loss of Nav1.6 channels impairs neuronal excitability and burst generation.

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Area of Science:

Neuroscience
Electrophysiology
Molecular Biology

Background:

Subthreshold sodium currents are implicated in rhythmic burst generation in mesencephalic trigeminal (Mes V) neurons.
Pharmacological studies suggest their critical role, but the specific contributions of different sodium current types remain unclear.

Purpose of the Study:

To characterize transient (INaT), persistent (INaP), and resurgent (Ires) sodium currents in Mes V neurons.
To investigate the functional consequences of lacking the Nav1.6 sodium channel subunit on Mes V neuron electrogenesis and burst firing.

Main Methods:

Utilized Na(v)1.6-null (med mouse, Na(v)1.6(-/-)) and wild-type (Na(v)1.6(+/+)) littermate mice.
Performed whole-cell patch-clamp recordings to measure sodium currents and assess neuronal excitability using current clamp (ZAP function).

Main Results:

Peak INaT, INaP, and Ires were significantly reduced in med mice (18%, 39%, 76% reduction, respectively).
Med neurons exhibited attenuated subthreshold oscillations and reduced resonant Q-value despite similar passive membrane properties.
Spike discharge was impaired, with reduced frequencies, spike block at low currents, and a complete absence of stimulus-induced rhythmical burst discharge.

Conclusions:

Subthreshold sodium currents, especially resurgent currents, are critical determinants of Mes V neuron electrogenesis and burst generation.
Nav1.6 channels play a significant role in controlling Mes V neuron excitability and rhythmic bursting behavior.