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Updated: Jul 14, 2026
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
A simple and practical agglutination assay for the detection of human anti-mouse antibodies
Ines Socher1, Oliver Meyer, Tunay Aslan
1Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany.
Background And Objectives:
Human anti-mouse antibodies (HAMAs) are relatively common in human serum and may interfere with therapeutic and diagnostic mouse monoclonal antibodies (MoAbs). We developed a simple particle agglutination test (PaGIA) for the detection of HAMAs.
Design And Methods:
Red-dyed high density particles were coated with monoclonal mouse IgG. These particles were incubated in the reaction chamber of a gel-card together with serum samples obtained from healthy blood donors (n=32), and patients with clinically proven autoimmune thrombocytopenia (AITP; n=26). Positive reactions were defined by a layer of particles on top of the gel or agglutinated particles dispersed throughout the gel matrix. Furthermore, MoAb-coated particles were subjected to flow cytometry and the results were compared with the new HAMA PaGIA.
Results:
HAMAs were detectable in 33% of serum samples tested (n=58). Results from flow cytometric analysis revealed a high parallel to those obtained by the PaGIA. Interestingly, we observed an increased incidence of HAMAs in AITP patients (42%) compared to healthy blood donors (26%).
Interpretation And Conclusion:
The new HAMA PaGIA allows a specific and easy, rapid detection of HAMAs and is suitable for large scale testing.
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