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Terezia Salaj1, Bart Panis, Rony Swennen

  • 1Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic. nrgrtesa@savba.sk

Cryo Letters
|May 25, 2007
PubMed

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...