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Updated: Jul 14, 2026

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope
Published on: June 20, 2019
Larch (Larix kaempferi) xylem parenchyma cells respond to subfreezing temperature by deep supercooling
Jun Kasuga1, Naoki Takata, Kenichi Yamane
1Laboratory of Woody Plant Biology, Hokkaido University, Sapporo, Japan.
Abstract:
In previous studies, xylem parenchyma cells (XPCs) in the boreal softwood species larch, which has thick and rigid walls similar to those of XPCs in boreal hardwood species, were shown to respond to subfreezing temperature by deep supercooling during summer but change their freezing behavior to extracellular freezing during winter. In this study, we re-examined freezing behavior of XPCs in larch by observation of deep etching of frozen samples as well as observation of re-warmed samples after freezing using a cryo-scanning electron microscope. The results showed that XPCs in larch adapts to subfreezing temperature by deep supercooling throughout all seasons. Such freezing behavior is the same as that of XPCs in boreal hardwood species.
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