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Phage typing of Staphylococcus saprophyticus.

A Torres Pereira1, J A Melo Cristino

  • 1Instituto Bacteriológico Câmara Pestana, University of Lisbon, Portugal.

Epidemiology and Infection
|December 1, 1991
PubMed
Summary

This study developed a phage typing system for Staphylococcus saprophyticus, identifying 26 lysogenic strains. This method aids in tracing urinary tract infection origins and complements plasmid profiling for strain differentiation.

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Area of Science:

  • Microbiology
  • Bacteriology
  • Virology

Background:

  • Staphylococcus saprophyticus is a common cause of urinary tract infections (UTIs).
  • Accurate strain differentiation is crucial for epidemiological studies and infection source tracing.
  • Existing typing methods may lack sufficient discriminatory power.

Purpose of the Study:

  • To develop and evaluate a phage typing system for Staphylococcus saprophyticus.
  • To assess the typability and reproducibility of the phage typing system.
  • To explore the potential of phage typing for epidemiological investigations of S. saprophyticus.

Main Methods:

  • Isolation and characterization of 502 Staphylococcus strains, including S. saprophyticus.
  • Mitomycin C induction of S. saprophyticus isolates to identify lysogenic strains.
  • Phage propagation, titration, and cross-streaking to establish lytic groups.
  • Evaluation of typability and reproducibility of the phage typing system.

Main Results:

  • Twenty-six out of 100 induced S. saprophyticus strains were found to be lysogenic.
  • Twelve phages formed three lytic groups (A, B, C), encompassing 75% of typable strains.
  • The phage typing system demonstrated 45% typability and high reproducibility (94.2%-100%).
  • Phages primarily lysed S. saprophyticus and rare novobiocin-resistant species, not S. aureus or S. epidermidis.

Conclusions:

  • Phage typing is an effective method for differentiating Staphylococcus saprophyticus strains.
  • This system can aid in ecological studies and tracing the origin of UTIs from skin or mucous membranes.
  • Phage typing, combined with plasmid profiling, offers strong discriminatory power for S. saprophyticus strain analysis.

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