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[Genetic markers of pathogenicity of conditionally pathogenic enterobacteria isolated from children with diarrheal

N Iu Zherebtsova, A Kh Baĭmiev, D A Valishin

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |May 26, 2007
    PubMed

    Insights

    Pathogenicity island genes in Enterobacteriaceae bacteria were investigated in children with diarrhea. Some bacterial isolates carried virulence genes, suggesting a role in diarrheal disease development.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Context:

    • Diarrheal diseases pose a significant health challenge, particularly in children.
    • Enterobacteriaceae family bacteria are common causes of infectious diarrhea.
    • Pathogenicity islands (PAIs) are mobile genetic elements contributing to bacterial virulence.

    Purpose:

    • To investigate the prevalence of specific pathogenicity island (PAI) genes in Enterobacteriaceae isolates from children with diarrhea.
    • To identify correlations between PAI genes and antibiotic resistance patterns.

    Summary:

    • Eighty-nine Enterobacteriaceae isolates (including Klebsiella spp., Proteus spp., and Morganella morganii) were analyzed from children with diarrhea.
    • Polymerase chain reaction (PCR) detected PAI gene fragments in 32.6% of isolates, including hlyA, hlyB, sfaG, cnf1, and estB.
    • Positive correlations were observed between hlyB and cnf1, and between hlyA and sfaG. sfaG showed a weak positive correlation with tetracycline and chloramphenicol resistance.

    Impact:

    • Identifies specific virulence factors in Enterobacteriaceae that may contribute to diarrheal disease severity.
    • Provides insights into the genetic basis of bacterial pathogenicity in pediatric diarrhea.
    • May inform strategies for diagnosing and managing infectious diarrhea caused by these bacteria.

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