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Published on: November 23, 2015
Cholesterol biosensor based on amino-undecanethiol self-assembled monolayer using surface plasmon resonance technique
Pratima R Solanki1, Sunil K Arya, Y Nishimura
1Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India.
Langmuir : the ACS Journal of Surfaces and Colloids
|May 26, 2007
Summary
This study developed a novel cholesterol biosensor by immobilizing cholesterol oxidase onto a gold surface. The resulting biosensor accurately detects cholesterol levels and demonstrates good reusability and shelf life.
Area of Science:
- Biochemistry
- Materials Science
- Electrochemistry
Background:
- Cholesterol detection is crucial for diagnosing various health conditions.
- Developing sensitive and stable biosensors is essential for accurate cholesterol monitoring.
- Immobilization of enzymes on suitable substrates enhances biosensor performance.
Purpose of the Study:
- To develop a novel cholesterol biosensor using immobilized cholesterol oxidase.
- To characterize the performance of the developed bioelectrode for cholesterol estimation.
- To evaluate the stability and reusability of the cholesterol biosensor.
Main Methods:
- Covalent immobilization of cholesterol oxidase (ChOx) onto 11-amino-1-undecanethiol hydrochloride (AUT) self-assembled monolayer (SAM) on gold (Au) substrates.
- Fabrication of ChOx/AUT/Au bioelectrodes using glutaraldehyde cross-linker.
- Characterization using contact angle (CA) measurements, electrochemical techniques, and atomic force microscopy (AFM).
- Cholesterol estimation in solution using surface plasmon resonance (SPR) technique.
Main Results:
- The developed bioelectrodes exhibited linearity for cholesterol detection from 50 to 500 mg/dL.
- A sensitivity of 1.23 m0/(mg dL) was achieved for cholesterol estimation.
- The biosensors demonstrated reusability for over 20 cycles.
- The shelf life was found to be approximately 10 weeks when stored at 4°C.
Conclusions:
- The developed ChOx/AUT/Au bioelectrode is a promising platform for sensitive and stable cholesterol detection.
- The immobilization strategy provides a robust method for creating effective cholesterol biosensors.
- The sensor's performance characteristics suggest its potential for practical diagnostic applications.
