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Updated: Jul 14, 2026

Intracellular Refolding Assay
Published on: January 24, 2012
[Heat-shock protein 27 linked to multi-drug resistance in human hepatic cancer cell lines]
Shi-fang Peng1, Lei Fu, De-ming Tan
1Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China. shifang70peng@yahoo.com
Objective:
A proteomics approach was applied for finding multi-drug resistance (MDR) related proteins in hepatic cancer cell lines.
Methods:
Two-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant hepatic cancer cell line HepG2/VCR and its counterpart HepG2. The differential expression proteins between the two cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Heat-shock protein 27 (HSP27), one of the differential expression proteins in the development of MDR of HepG2/VCR, was analyzed by HSP27 antisense oligonucleotides (ASOs).
Results:
As a high expression protein in HepG2/VCR, HSP27 was identified, and the suppression of HSP27 expression by HSP27 ASO enhanced the vincristine chemosensitivity of HepG2/VCR (P less than 0.05).
Conclusion:
HSP27 is linked to MDR in human hepatic cancer cell line HepG2/VCR.
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