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Updated: Jul 14, 2026

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
Published on: December 26, 2017
Exercise-induced liver beta2-microglobulin expression is related to lower IgG clearance in the blood
Koutarou Suzuki1, Park Jun Suk, Chunlan Hong
1Doctoral Program in Health and Sport Sciences, Postgraduate School of Comprehensive Human Sciences, University of Tsukuba, Japan.
Abstract:
Voluntary wheel running exercise induced higher antigen-specific IgG in circulating blood is well recognized in mice. This antibody response may be regulated by an exercise-induced mechanism that protects against IgG catabolism. The recent hypothesis that the beta2-microglobulin gene is implicated in IgG protection is investigated further on mice voluntary wheel running. Male C57BL/6N mice were intraperitoneally immunized with 0.375microg/kg (body weight) of tetanus toxoid to induce primary and secondary antibody responses. At the peak concentration of blood tetanus toxoid specific IgG in this experiment, we administered (125)I-labeled mouse IgG. To determine how (125)I-IgG half-life is prolonged in voluntary wheel running exercised mice, we observed the tissue radioactivity (125)I-IgG. Significantly higher blood IgG concentrations were demonstrated in the exercised group compared to non-exercised group (P<.05). The mean value of radioactivity in the liver was higher in the exercised group (P<.05). Furthermore, extracted IgG concentration of exercised mouse liver was higher than that of non-exercised group (P<.05). Immunohistochemical analysis showed dramatically increased tissue IgG in the liver of the exercised group (P<.05). The gene expression of beta2-microglobulin was up-regulated in the exercised mouse liver (P<.05). There is a significant correlation between liver accumulation of (125)I-IgG and (125)I-IgG concentration in the blood (P<.05). In addition, there is a significant correlation between extracted total hepatic IgG and beta2-microglobulin in the liver (P<.05). These findings indicate that voluntary wheel running exercise-induced liver beta2-microglobulin expression is related to lower IgG clearance in the blood.
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