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Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Published on: July 27, 2022

Recommendations for improved standardization of immunohistochemistry.

Neal S Goldstein¹, Stephen M Hewitt, Clive R Taylor

¹Department of Anatomic Pathology, William Beaumont Hospital, Royal Oak, MI 48073, USA. NGoldstein@beaumont.edu

Applied Immunohistochemistry & Molecular Morphology : AIMM

|May 26, 2007

Summary

Standardizing immunohistochemistry (IHC) is crucial for reliable results. This report outlines recommendations to improve consistency and reproducibility in IHC assays by addressing fixation and validation issues.

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Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Area of Science:

Biomedical diagnostics
Histopathology
Laboratory medicine

Background:

Immunohistochemistry (IHC) assays frequently exhibit inconsistent results, poor reproducibility, and quality control issues.
Lack of standardization leads to challenges in concordance, validation, and verification of IHC findings.

Framework:

Recommendations from the Ad-Hoc Committee on Immunohistochemistry Standardization are presented.
Identifies underfixation, irregular fixation, and non-standard fixatives as key contributing factors.
Highlights the absence of thorough IHC assay optimization, validation, and standardized interpretation systems.

Implementation:

Provides definitions and detailed guidelines for addressing fixation variability.
Emphasizes the need for understanding IHC assay parameters.
Recommends establishing standardized interpretation and reporting systems.

Implications:

Aims to improve the reliability and consistency of immunohistochemistry results.
Enhances the quality assurance and validation of diagnostic pathology.
Facilitates better inter-laboratory concordance and clinical decision-making.