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Evaluation of a method for simultaneous quantification of codeine, ethylmorphine and morphine in blood
H Gjerde1, U Fongen, H Gundersen
1National Institute of Forensic Toxicology, Oslo, Norway.
Forensic Science International
|October 1, 1991
Summary
A new method accurately quantifies common opiates like codeine, ethylmorphine, and morphine in forensic blood samples. This technique offers low detection limits and high precision for reliable opiate analysis.
Area of Science:
- Forensic Toxicology
- Analytical Chemistry
- Pharmacology
Background:
- Codeine, ethylmorphine, and morphine are frequently identified opiates in Norwegian forensic blood analyses.
- Accurate quantification of these opiates is crucial for forensic investigations.
Purpose of the Study:
- To evaluate a novel method for the simultaneous quantification of codeine, ethylmorphine, and morphine.
- To assess the method's performance in forensic blood sample analysis.
Main Methods:
- Solid phase extraction (SPE) for sample preparation.
- Gas chromatography-mass spectrometry (GC-MS) for simultaneous opiate detection and quantification.
Main Results:
- Achieved low detection limits: codeine (0.026 µmol/l), ethylmorphine (0.025 µmol/l), and morphine (0.032 µmol/l).
- Demonstrated high analytical precision with variations below 5% at relevant concentrations.
Conclusions:
- The developed SPE-GC-MS method is suitable for the simultaneous quantification of codeine, ethylmorphine, and morphine in forensic blood samples.
- The method provides sensitive and precise results, aiding in forensic opiate analysis.