Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Force-response considerations in ciliary mechanosensation.

Andrew Resnick1, Ulrich Hopfer

  • 1Department of Physiology and Biophysics, Case Western Reserve School of Medicine, Cleveland, Ohio 44106, USA. andy.resnick@case.edu

Biophysical Journal
|May 29, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Rat Strain Effects of AT1 Receptor Activation on D1 Dopamine Receptors in Immortalized Renal Proximal Tubule Cells.

Hypertension (Dallas, Tex. : 1979)·2025
Same author

Correction: Altered AT1 Receptor Regulation of ETB Receptors in Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats.

Hypertension (Dallas, Tex. : 1979)·2025
Same author

Microstructure-based modeling of primary cilia mechanics.

Cytoskeleton (Hoboken, N.J.)·2024
Same author

Microstructure-Based Modeling of Primary Cilia Mechanics.

bioRxiv : the preprint server for biology·2023
Same author

Fluid-structure interaction modelling of neighboring tubes with primary cilium analysis.

Mathematical biosciences and engineering : MBE·2023
Same author

Sources of exposure identified through structured interviews of healthcare workers who test positive for severe acute respiratory coronavirus virus 2 (SARS-CoV-2): A prospective analysis at two teaching hospitals.

Antimicrobial stewardship & healthcare epidemiology : ASHE·2022
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
Same journal

Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

Biophysical journal·2026
See all related articles

Renal collecting duct chief cells

Area of Science:

  • Cell Biology
  • Biophysics
  • Physiology

Background:

  • Primary cilia function as mechanosensory organelles in epithelial cells.
  • The precise relationship between cellular responses and mechanical forces on cilia is not fully understood.

Purpose of the Study:

  • To investigate the effects of mechanical forces on renal collecting duct chief cells.
  • To quantify the forces exerted on cilia during orbital shaking.
  • To determine the role of cilia in mechanosensation in these cells.

Main Methods:

  • Exposure of renal collecting duct chief cell monolayers to controlled orbital shaking.
  • Measurement of amiloride-sensitive sodium current.
  • Quantification of ciliary length and estimation of drag forces on cilia.

Related Experiment Videos

Main Results:

  • 24-hour orbital shaking decreased amiloride-sensitive sodium current by ~60% and ciliary length by ~30%.
  • Shaking-induced reduction in sodium current was dependent on intact cilia.
  • Estimated maximal drag force on cilia was 5.2x10(-3) pN, significantly exceeding thermal noise.

Conclusions:

  • The primary cilium acts as a mechanosensory organelle in renal collecting duct chief cells.
  • Ciliary length is a key factor in the cell's mechanosensory response.
  • Cilia exhibit a lower mechanosensory sensitivity than previously recognized.