Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SIMPLE approach for isolating mutants expressing fimbriae.

Sean-Paul Nuccio1, Daniela Chessa, Eric H Weening

  • 1Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, Davis, CA 95616-8645, USA.

Applied and Environmental Microbiology
|May 29, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does gut oxygenation drive colorectal cancer?

Molecular cell·2026
Same author

Predicting competition and substrate preferences for targeted microbiome alteration.

Cell·2026
Same author

Copper is an intestinal habitat filter affecting the gut microbiota interactions with Salmonella Typhimurium.

Microbiome·2026
Same author

Host-derived nitrate fuels indole production by <i>Escherichia coli</i> to drive chronic kidney disease progression.

Science (New York, N.Y.)·2026
Same author

A searchable metadata network graph for microbiome metabolomics.

bioRxiv : the preprint server for biology·2026
Same author

Siderophore-Pt(IV) Conjugates as Tools to Probe Cytoplasmic Cargo Delivery to Gram-Negative Bacteria.

Journal of the American Chemical Society·2026
Same journal

A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.

Applied and environmental microbiology·2026
Same journal

Sources and traits of bacteria and fungi found in the near-surface atmosphere.

Applied and environmental microbiology·2026
Same journal

Advancing One Health in Africa through continental early warning environmental surveillance.

Applied and environmental microbiology·2026
Same journal

Specificity and longevity of a bacterial interspecies mutual cooperation benefiting organic micropollutant biodegradation.

Applied and environmental microbiology·2026
Same journal

Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.

Applied and environmental microbiology·2026
Same journal

Synergistic and individual effects of RNase E, II, and R in the regulation of <i>Escherichia coli</i> growth and metabolism.

Applied and environmental microbiology·2026
See all related articles

Researchers developed a new method, Screening with Immunomagnetic Particles for Ligand Expression (SIMPLE), to identify fimbriated bacteria. This technique successfully identified previously unexpressed fimbrial operons in Salmonella Typhimurium.

Area of Science:

  • Microbiology
  • Bacterial genetics
  • Molecular biology

Background:

  • Enterobacteriaceae genomes possess numerous fimbrial operons, but many are poorly expressed in vitro.
  • Functional characterization of fimbriae is hindered by challenges in inducing their expression.

Purpose of the Study:

  • To develop and validate a novel method for identifying bacteria expressing fimbriae.
  • To facilitate the functional characterization of poorly expressed fimbrial operons.

Main Methods:

  • Development of the Screening with Immunomagnetic Particles for Ligand Expression (SIMPLE) technique.
  • Application of SIMPLE to screen a library of Salmonella Typhimurium transposon mutants.
  • Identification of mutants expressing PefA fimbriae.

Related Experiment Videos

Main Results:

  • The SIMPLE method successfully identified four T-POP mutants of Salmonella Typhimurium expressing PefA fimbriae.
  • These mutants contained transposon insertions in the hns gene, which encodes a global silencer.
  • The pef fimbrial operon, typically unexpressed in Luria-Bertani broth, was identified.

Conclusions:

  • The SIMPLE method is an effective approach for isolating fimbriated bacteria.
  • This technique can be readily applied to fimbrial operons identified through whole-genome sequencing.