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Updated: Jul 14, 2026

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Published on: August 6, 2020

Endogenous endostatin inhibits choroidal neovascularization.

Alexander G Marneros¹, Haicheng She, Hadi Zambarakji

¹Department of Developmental Biology, Harvard School of Dental Medicine, 188 Longwood Ave., Boston, MA 02115, USA. alexander_marneros@yahoo.com

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology

|May 29, 2007

Summary

Endogenous endostatin inhibits pathological angiogenesis, like choroidal neovascularization (CNV). Supplementing endostatin significantly reduced CNV growth and leakage in mice, suggesting therapeutic potential.

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Area of Science:

Ophthalmology
Molecular Biology
Pathology

Background:

Endostatin, derived from collagen XVIII, shows antiangiogenic effects at high doses.
The role of endogenous endostatin at physiological levels in inhibiting pathological angiogenesis, such as choroidal neovascularization (CNV), remains unclear.

Purpose of the Study:

To investigate the role of endogenous endostatin in inhibiting pathological angiogenesis, specifically CNV.
To evaluate the therapeutic potential of endostatin in treating CNV.

Main Methods:

Induced CNV in mice lacking collagen XVIII/endostatin and in control mice using a laser injury model.
Administered recombinant endostatin to deficient and control mice.
Performed ultrastructural analysis of choroidal vasculature.

Main Results:

CNV lesions in mice lacking endostatin were approximately 3-fold larger with increased vascular leakage compared to controls.
Recombinant endostatin administration reduced CNV lesion size and vascular leakage in deficient mice.
Recombinant endostatin almost completely inhibited CNV in control mice.

Conclusions:

Endogenous endostatin acts as an inhibitor of induced angiogenesis.
Exogenous endostatin potently inhibits CNV growth and vascular leakage.
Endostatin holds therapeutic promise for inhibiting CNV pathogenesis.