Related Experiment Video
Updated: Jul 14, 2026
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
Published on: May 13, 2020
A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates
Tanya M Leavy1, Carolyn R Bertozzi
1Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA.
Abstract:
O-GlcNAc transferase (OGT) catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto a diverse array of intracellular proteins. Although hundreds of proteins are known to be modified by O-GlcNAc, a strict amino acid consensus sequence for OGT has not been identified. In this study, we describe the development of a high-throughput assay for OGT and use it to profile the specificity of the enzyme among a panel of peptide substrates.
