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Related Experiment Video

Updated: Jul 14, 2026

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

Published on: May 13, 2021

The ATR pathway: fine-tuning the fork.

Renee D Paulsen¹, Karlene A Cimprich

¹Department of Chemical and Systems Biology, Stanford University, 318 Campus Drive, Stanford, CA 94305-5441, USA.

|May 29, 2007

Summary

The ATR pathway is crucial for stabilizing stalled DNA replication forks, preventing genomic instability and cancer. Its proper function ensures DNA repair and cell cycle regulation.

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Genomic stability is vital and threatened during DNA replication by various damaging events.
Replication fork stalling can lead to DNA damage, genomic instability, and cancer.
The cell utilizes a replication checkpoint to manage stalled forks.

Purpose of the Study:

To review the mechanisms by which the ATR pathway recognizes and stabilizes stalled replication forks.
To highlight the importance of ATR and Chk1 in the replication checkpoint.

Main Methods:

This review synthesizes current research on the ATR pathway and replication checkpoint.
Focuses on the molecular mechanisms of fork stabilization.

Main Results:

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Last Updated: Jul 14, 2026

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

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Published on: May 13, 2021

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Published on: February 22, 2018

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The ATR-Chk1 pathway is central to the replication checkpoint, blocking cell cycle progression and stabilizing forks.
Loss of ATR or Chk1 leads to replication fork collapse and chromosomal instability.
ATR pathway activation is essential for preventing genomic rearrangements.

Conclusions:

The ATR pathway plays a critical role in maintaining genomic integrity by managing stalled replication forks.
Dysregulation of ATR signaling can predispose individuals to cancer.
Understanding ATR's role is key to developing therapeutic strategies for replication stress-induced diseases.