Related Experiment Video

Updated: Jul 14, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Complete coding sequences and phylogenetic analysis of Human Bocavirus (HBoV)

Thaweesak Chieochansin1, Salin Chutinimitkul, Sunchai Payungporn

  • 1Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Virus Research
|May 29, 2007
PubMed

Insights

Human Bocavirus (HBoV) causes respiratory illness in children. This study found HBoV in 7.14% of samples and sequenced positive cases, identifying conserved NS1 and NP1 genes for improved detection.

Area of Science:

  • Virology
  • Pediatric Infectious Diseases
  • Molecular Diagnostics

Background:

  • Human Bocavirus (HBoV) is an emerging pathogen linked to respiratory tract infections in infants and children.
  • Global surveillance studies have indicated a significant prevalence of HBoV in pediatric populations.

Purpose of the Study:

  • To determine the prevalence of HBoV in nasopharyngeal samples from children with respiratory illness.
  • To sequence and analyze the complete coding regions of HBoV to identify conserved genetic markers for improved detection.

Main Methods:

  • Polymerase Chain Reaction (PCR) was used to detect HBoV in 252 nasopharyngeal aspirates.
  • Whole genome sequencing of three positive samples was performed using a novel set of conserved primers.

Main Results:

  • HBoV was detected in 7.14% (18/252) of the pediatric samples.
  • The NS1 and NP1 genes exhibited high conservation across HBoV strains.
  • VP1 and VP2 genes showed significant variability, but variations were independent of geographical origin.

Conclusions:

  • The identified conserved regions, particularly NS1 and NP1, can enhance the development of robust HBoV detection methods.
  • The complete coding sequences provide valuable data for improving laboratory diagnostics and understanding HBoV molecular epidemiology.

More Related Videos

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes08:56

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Related Concept Videos

Bacteriophages of the Human Virome01:23

Bacteriophages of the Human Virome

Bacteriophages are found throughout the human body. They may even outnumber eukaryotic viruses, forming an important and dynamic component of the human virome. Indeed, phages represent the most abundant viral entities, with densities in the gut reaching up to 10⁹ particles per gram of fecal matter, and many belonging to orders such as Caudovirales and Microviridae, while a substantial proportion remains unclassified as viral “dark matter.”Lysogeny and Genetic ExchangeIn the gut, bacteriophages...
Human Virome01:26

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
Phylogeny01:23

Phylogeny

Phylogeny is concerned with the evolutionary diversification of organisms or groups of organisms. A group of organisms with a name is called a taxon (singular). Taxa (plural) can span different levels of the evolutionary hierarchy. For instance, the group containing all birds is a taxon (comprising the class Aves), and the group of all species of daisies (the genus Bellis) is a taxon. Phylogenies can likewise include just one genus (i.e., depict species relationships) or span an entire...
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...