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Updated: Jul 14, 2026
12:09
Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
Published on: December 31, 2013
TRPV6 channel controls prostate cancer cell proliferation via Ca(2+)/NFAT-dependent pathways
V Lehen'kyi1, M Flourakis, R Skryma
1Inserm, U-800, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, France.
Oncogene
|May 30, 2007
Summary
Transient Receptor Potential Vanilloid 6 (TRPV6) channels are upregulated in prostate cancer, controlling cell proliferation and survival. This study reveals TRPV6
Area of Science:
- Oncology
- Molecular Biology
- Channelopathies
Background:
- Transient Receptor Potential Vanilloid 6 (TRPV6) expression is elevated in advanced prostate cancer, correlating with higher Gleason scores.
- TRPV6 is a highly calcium-selective ion channel, but its specific role in prostate carcinogenesis is not well understood.
Purpose of the Study:
- To investigate the role of TRPV6 in prostate cancer cell proliferation, cell cycle progression, and apoptosis.
- To elucidate the downstream signaling pathways activated by TRPV6-mediated calcium influx in prostate cancer cells.
- To examine the regulation of TRPV6 expression by androgen receptor in prostate cancer.
Main Methods:
- Utilized LNCaP cell line, a human prostate cancer cell line.
- Assessed cell proliferation rates, cell cycle distribution (S-phase accumulation), and proliferating cell nuclear antigen (PCNA) expression.
- Measured calcium (Ca2+) uptake and activation of nuclear factor of activated T-cell transcription factor (NFAT).
- Investigated androgen receptor-dependent regulation of TRPV6.
Main Results:
- TRPV6 expression was found to decrease LNCaP cell proliferation rate, S-phase accumulation, and PCNA expression.
- TRPV6 mediates Ca2+ uptake into LNCaP cells, leading to downstream NFAT activation.
- TRPV6-mediated Ca2+ influx contributes to apoptosis resistance in LNCaP cells.
- TRPV6 expression in LNCaP cells is regulated by androgen receptor in a ligand-independent manner.
Conclusions:
- TRPV6 plays a significant role in controlling prostate cancer cell proliferation and survival.
- Upregulation of TRPV6 in prostate cancer cells may promote increased proliferation, cell survival, and apoptosis resistance.
- TRPV6-mediated calcium signaling is a potential therapeutic target in prostate cancer.
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