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Related Experiment Video

Updated: Jul 14, 2026

Method for the Isolation of Francisella tularensis Outer Membranes
11:06

Method for the Isolation of Francisella tularensis Outer Membranes

Published on: June 29, 2010

Immunodominant Francisella tularensis antigens identified using proteome microarray.

Jim E Eyles1, Berkay Unal, Margaret Gill Hartley

  • 1Defence Science and Technology Laboratory, Porton Down, Salisbury, UK. jeeyles@dstl.gov.uk

Proteomics
|May 30, 2007
PubMed
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Developing effective non-replicating vaccines against intracellular pathogens like Francisella tularensis is challenging. This study identified novel immunoreactive antigens and revealed insights into protective immune responses, aiding future vaccine design.

Area of Science:

  • Immunology
  • Vaccinology
  • Microbiology

Background:

  • Stimulating robust immune responses against intracellular pathogens with non-replicating vaccines is a significant challenge.
  • Francisella tularensis (tularemia) poses a substantial biodefense threat, necessitating effective vaccine strategies.

Purpose of the Study:

  • To identify immunodominant antigens of Francisella tularensis using a whole proteome microarray.
  • To gain insights into the protective immune responses elicited by a non-replicating vaccine candidate.
  • To inform the rational design of novel vaccines against tularemia and other intracellular pathogens.

Main Methods:

  • BALB/c mice were immunized with killed F. tularensis (LVS) vaccine adjuvanted with immune-stimulating complexes and CpG.
  • Protective immunity was assessed following challenge with virulent F. tularensis (Schu S4).

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Last Updated: Jul 14, 2026

Method for the Isolation of Francisella tularensis Outer Membranes
11:06

Method for the Isolation of Francisella tularensis Outer Membranes

Published on: June 29, 2010

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
08:52

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Published on: July 26, 2019

  • Serum from immunized mice was used to probe a whole proteome microarray to identify immunoreactive antigens.

    • Main Results:

    • Immunization conferred protection against virulent F. tularensis challenge.
    • The proteome microarray identified 11 known and 31 novel immunoreactive antigens.
    • Surface/membrane-associated proteins and hypothetical proteins were significantly represented among immunodominant antigens.
    • IgG subclass bias in protected versus unprotected animals was determined.

    Conclusions:

    • The study successfully identified novel immunoreactive antigens for Francisella tularensis.
    • Proteome-wide antigen discovery provides valuable data for developing subunit or non-living vaccines.
    • Understanding antigen-specific immune responses is crucial for designing effective vaccines against intracellular pathogens.