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Updated: Jun 25, 2026
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
[Osteoblastic differentiation of goat bone marrow stromal cells after AdBMP-2 mediated transduction in vitro]
1College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, China.
Purpose:
To investigate the effects of adenuvirus mediated transduction with bone murphogenetic protein-2 on proliferation and osteoblastic differentiation of goat bone marrow stromal cells.
Methods:
Adult goat bone marrow stromal cells were separated and cultured in vitro and transduced with either AdBMP-2 or AdLacZ gene. The transfer efficiency was calculated under microscopy. Cell proliferation was described by growth curve. Alkaline phosphatase staining and quantitative osteocalcin expression were detected after gene transduction.The data were analyzed by SAS6.2 software package with SNK test.
Results:
The transfer efficiency reached 70% +/- 3%. No significant difference was found in cell proliferation among 3 groups. Alkaline phosphatase staining was more pronounced and osteocalcin expression was enhanced in AdBMP-2 transfered group.There was significant difference in osteocalcin expression between AdBMP-2 transferred group and the control group (P < 0.01).
Conclusion:
Adenovirus mediated transduction with bone morphogenetic protein-2 enhanced their osteogenic capacity in vitro which might be an alternative cell source for maxillofacial bone regeneration. Supported by National Natural Science Foundation of China (Grant No.30400502), Science and Technology Commission of Shanghai Municipality (Grant No.04dz05601, 05DJ14006, 055407034, 05QMoX14o6) and Research Grant from Shanghai Education Committee (Grant No. 03BC39, 04YQHB081, Y0203).
