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Updated: Jul 14, 2026
08:52
Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
Published on: February 22, 2019
Genetic control of host-pathogen interactions in mice
Gundula Min-Oo1, Mary M Stevenson, Anny Fortin
1Department of Biochemistry, McGill University, Montreal, Canada.
Novartis Foundation Symposium
|May 31, 2007
Summary
Genetic analysis in mouse models reveals key host genes controlling infection outcomes. Discoveries include Nrampl for Mycobacterium, Birc1e/Naip5 for Legionella, and new Plasmodium loci.
Area of Science:
- Immunology
- Genetics
- Microbiology
Background:
- Host defense mechanisms and pathogen virulence determine infection outcomes.
- Genetic analysis in mice identifies host genes crucial for host-pathogen interactions.
- Variations in mouse strains reveal genetic effects on infection susceptibility.
Purpose of the Study:
- To discover host genes and proteins essential for macrophage function against bacterial and parasitic infections using mouse models.
- To elucidate the genetic basis of host resistance and susceptibility to pathogens like Mycobacterium, Legionella, and Plasmodium.
Main Methods:
- Utilizing mouse models of infection with Mycobacterium, Legionella, and Plasmodium.
- Employing genetic mapping techniques including positional cloning to identify relevant genes.
- Analyzing macrophage function in response to infectious agents.
Main Results:
- Identified Nrampl-mediated divalent metal exclusion from phagosomes as critical for controlling Mycobacterium replication.
- Demonstrated that functional Birc1e/Naip5 protein is essential for intracellular sensing and inhibition of Legionella replication in macrophages.
- Discovered and cloned two new genetic loci influencing blood-stage Plasmodium chabaudi AS replication in mice.
Conclusions:
- Host genetic factors significantly impact the control of intracellular bacterial and parasitic infections.
- Specific genes like Nrampl and Birc1e/Naip5 play vital roles in macrophage-mediated host defense.
- Genetic studies in mice provide powerful insights into host-pathogen interactions and potential therapeutic targets.
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