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Updated: Jul 14, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
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Steroid receptor phosphorylation: a key modulator of multiple receptor functions.

Nancy L Weigel1, Nicole L Moore

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA. nweigel@bcm.tmc.edu

Molecular Endocrinology (Baltimore, Md.)
|May 31, 2007
PubMed
Summary

Steroid receptor phosphorylation, a key posttranslational modification, integrates cell signaling pathways. Site-specific phosphorylation regulates receptor activity, influencing hormone response, DNA binding, and gene expression.

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Published on: September 21, 2011

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Endocrinology

Background:

  • Steroid receptors are crucial transcription factors regulated by hormone activation.
  • Posttranslational modifications, particularly phosphorylation, significantly impact steroid receptor expression and activity.
  • Numerous kinases and phosphorylation sites suggest complex integration with cellular signaling networks.

Purpose of the Study:

  • To review current knowledge on steroid receptor phosphorylation.
  • To elucidate the regulatory roles of site-specific phosphorylation in steroid receptor function.
  • To highlight the integration of cell-signaling pathways with steroid receptor action.

Main Methods:

  • Literature review focusing on phosphorylation sites and regulatory mechanisms.
  • Analysis of studies utilizing phosphorylation site-specific antibodies and advanced functional assays.
  • Synthesis of data on how phosphorylation impacts various aspects of receptor function.

Main Results:

  • Phosphorylation sites are prevalent across steroid receptor domains, predominantly in the N-terminus.
  • Site-specific phosphorylation regulates hormone sensitivity, DNA binding, stability, localization, and protein interactions.
  • Phosphorylation integrates diverse cell-signaling pathways, modulating target gene regulation.

Conclusions:

  • Steroid receptor phosphorylation is a critical regulatory mechanism with diverse, site-specific functions.
  • Understanding phosphorylation is essential for deciphering steroid receptor action and cellular signaling.
  • Advancements in assays will likely reveal further roles for phosphorylation in receptor regulation.