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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[Genotyping of genital human papillomavirus by DNA sequencing and luminex methods]
Shu-Zhen Qi1, Qian-Qiu Wang, Yu Tan
1STD Clinic, Institute of Dermatology, CAMS and PUMC, Nanjing 210042, China.
Objective:
To compare the specificity and sensitivity of two genotyping approaches for human papillomavirus (HPV).
Method:
HPV DNA was amplified and detected in clinical specimens by polymerase chain reaction in a pair of universal primers MY09/11, and then genotyped with either sequencing method or liquid chip hybridization method (luminex method).
Result:
Sequencing method obtained precise genotyping results in single-type HPV infection, while luminex method obtained accurate genotyping results in multiple-type HPV infection.
Conclusion:
A combined method using both sequencing and luminex method is suitable for the genotyping of HPV-infected specimens.
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