Effects of river otter activity on terrestrial plants in trophically altered Yellowstone Lake

Animals that deposit aquatically derived nutrients on terrestrial landscapes link food webs and affect a variety of in situ processes. This phenomenon, however, is poorly documented in freshwater habitats, especially where species introductions have drastically changed an ecosystem's trophic structure. In this study, we used stable isotopes to document water-to-land nutrient transport by river otters (Lontra canadensis) around Yellowstone Lake, an ecosystem recently altered by nonnative species invasions. We then investigated the effects of otter fertilization on plant growth and prevalence at latrine (scent-marking) sites and evaluated how the recent changes to the lake's food web could influence these plant responses. Values of delta15N were higher on latrines compared to non-latrine sites in five of seven sample plant taxa. Additionally, latrine grasses had higher percentage N than those from non-latrines. Foliar delta15N positively related to fecal deposition rate for some plants, indicating that increased otter scent-marking led to a rise in these N values. Logistic regression models indicated that otters selected for well-shaded latrines with access to foraging. Atypical latrines, misclassified as non-latrines by the regression models, had values of delta15N similar to correctly classified latrines, suggesting that site effects alone cannot explain elevated N values at otter latrine sites. No difference in plant diversity or percent cover of N-fixing taxa occurred between latrine and nonlatrine sites, though specific genera did differ between site types. Measurements of shoot lengths indicated increased growth of some latrine currants (Ribes sp.). In Yellowstone Lake, a twofold reduction in otter numbers could result in an even greater decline in nutrient deposition at latrines, as otters may become less social in a system with decreased prey availability. Our results highlight the role of animals in linking aquatic and terrestrial habitats in inland freshwater systems and suggest that ongoing changes in the trophic structure of Yellowstone Lake could have unexpected ramifications well beyond the lake itself.