Related Experiment Video
Updated: Jul 14, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
Published on: September 13, 2014
A comparative study of capillary zone electrophoresis and pH-potentiometry for determination of dissociation
Melinda Andrasi1, Peter Buglyo, Laszlo Zekany
1Department of Inorganic and Analytical Chemistry, University of Debrecen, PO Box 21, 4010 Debrecen, Hungary.
Abstract:
Acidity constants of six cephalosporin antibiotics, cefalexin, cefaclor, cefadroxil, cefotaxim, cefoperazon and cefoxitin are determined using capillary zone electrophoresis (CZE) and pH-potentiometric titrations. Since CZE is a separation method, it is not necessary for the samples to be of high purity and known concentration because only mobilities are measured. The effect on determination of dissociation constants of different matrices (serum, 0.9% NaCl, fermentation matrix) was examined. The advantages of CZE can be utilized in those fields where potentiometry has limitations (sample quantity, solubility, purity, simultaneous determinations), although pK(a) values that are close to each other can be determined by potentiometry with more accuracy.
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Potentiometry: Overview
Electrophoresis: Overview
There...
Capillary Electrophoresis: Instrumentation
Controlled-Potential Coulometry: Electrolytic Methods
The chosen potential ensures...
Potentiometric Titration: Overview

