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Related Experiment Videos

Progesterone binding in rabbit oviduct and uterus.

E K Muechler, G L Flickinger, L Mastroianni

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
    |February 1, 1976
    PubMed
    Summary

    Rabbit uterus and oviduct cytosol contain high-affinity progesterone receptors. Receptor levels are lower in the oviduct than the uterus and decline post-mating, with oviductal levels remaining higher during early pregnancy.

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    Area of Science:

    • Reproductive biology
    • Endocrinology
    • Molecular biology

    Background:

    • Progesterone plays a crucial role in reproductive processes.
    • Understanding progesterone receptor dynamics is essential for reproductive health.

    Purpose of the Study:

    • To characterize progesterone binding and receptor concentrations in the rabbit oviduct and uterus.
    • To investigate changes in progesterone receptor levels during estrus and early pregnancy.

    Main Methods:

    • Cytosol preparation from rabbit oviduct and uterus.
    • High-affinity progesterone binding assays.
    • Determination of sedimentation coefficients for receptor macromolecules.
    • Quantification of progesterone receptor concentration.

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    Main Results:

    • High-affinity progesterone binding (Kd = 10(-9) M) was detected in rabbit oviduct and uterine cytosol.
    • Receptor macromolecules exhibited sedimentation coefficients of 7-8 S and 4-5 S.
    • Progesterone receptor concentration was 2-5 fold lower in the oviduct compared to the uterus.
    • Uterine receptor concentration declined significantly from 3 to 144 hours post-mating.
    • Oviductal receptor concentration decline was not significant until day six of pregnancy.
    • Progesterone receptor levels exceeded estradiol receptor levels in both tissues during estrus and early pregnancy.

    Conclusions:

    • Rabbit oviduct and uterus possess high-affinity progesterone receptors.
    • Differential regulation of progesterone receptor levels exists between the oviduct and uterus during early pregnancy.
    • Progesterone receptors are more abundant than estradiol receptors in these tissues during estrus and early pregnancy.