Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes.

Benoit Palancade1, Xianpeng Liu, Maria Garcia-Rubio

  • 1Institut Curie, Centre de Recherche, and Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique, F-75248 Paris, France. palancad@crie.fr

Molecular Biology of the Cell
|June 1, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lymphatic dysfunction and ZFP36 deficiency contribute to myxomatous valve degeneration in Marfan Syndrome mice.

The Journal of clinical investigation·2026
Same author

Disruption of the structural maintenance of chromosomes 5/6 complex enables tumor mutagenesis.

NAR cancer·2026
Same author

SETD7-mediated epigenetic regulation of LncRNA XIST promotes trophoblast pyroptosis: a study in preeclampsia mouse and cell models.

Biology direct·2026
Same author

Multi-subunit collaboration enables Smc5/6 to function as a composite SUMO E3 complex.

Research square·2026
Same author

Replication fork remodeling proteins, Smc5/6 and Rtt107, promote palindrome-mediated genome instability.

bioRxiv : the preprint server for biology·2026
Same author

Correction: An essential gene screening identifies yeast Mot1 as a suppressor of R-loops and genome instability.

PLoS genetics·2026
Same journal

Mechanisms underpinning chromosome structure in metazoans.

Molecular biology of the cell·2026
Same journal

Conserved and Divergent Modes of Substrate Interaction Define Selective Localizations and Functions of a Cdc14 Phosphatase.

Molecular biology of the cell·2026
Same journal

Dimerization of the centriolin-like protein Nud1 governs spindle pole body inheritance in budding yeast.

Molecular biology of the cell·2026
Same journal

Non-muscle Myosin II acts as a negative feedback mediator to control cell contraction dynamics in adherent cells.

Molecular biology of the cell·2026
Same journal

The tetraspanin disc proteins, peripherin-2 and ROM1, facilitate CNG channel localization to the rod outer segment.

Molecular biology of the cell·2026
Same journal

Csf1 facilitates adaptive membrane lipid remodeling linked to ER-plasma membrane contact sites.

Molecular biology of the cell·2026
See all related articles

Nuclear pore complexes (NPCs) and their components, Nup84 and Nup60/Mlp1-2, are crucial for maintaining genome stability. These nucleoporins regulate protein sumoylation, essential for DNA repair processes and preventing double-strand breaks (DSBs).

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Nuclear pore complexes (NPCs) play multifaceted roles in nuclear metabolism, including transcription, organization, and DNA repair.
  • The Nup84 complex, a key NPC component, has been previously implicated in DNA repair pathways.

Purpose of the Study:

  • To investigate the shared function of the Nup84 and Nup60/Mlp1-2 complexes in relation to double-strand break (DSB) appearance.
  • To elucidate the molecular mechanism connecting NPCs to genome stability through protein sumoylation.

Main Methods:

  • Genetic interaction analysis of Nup84 and Nup60/Mlp1-2 complex mutants.
  • Assessment of DNA repair processes and double-strand break accumulation in nucleoporin mutants.
  • Analysis of Ulp1 (SUMO-protease) levels and protein sumoylation patterns, including the DNA repair factor Yku70.

Related Experiment Videos

Main Results:

  • Mutants of the Nup84 and Nup60/Mlp1-2 complexes exhibit similar genetic interactions and DNA repair defects as mutants of the SUMO-protease Ulp1.
  • These nucleoporins are essential for maintaining Ulp1 levels at NPCs and for proper sumoylation of key proteins like Yku70.
  • Restoring nuclear envelope-associated Ulp1 in nucleoporin mutants corrected sumoylation, suppressed DSB accumulation, and ameliorated genetic interactions.

Conclusions:

  • The Nup84 and Nup60/Mlp1-2 complexes share a functional link in preventing double-strand breaks (DSBs).
  • Nucleoporins regulate genome stability by controlling Ulp1 levels and protein sumoylation at the nuclear envelope.
  • This study reveals a molecular mechanism linking NPC function to genome stability via sumoylation regulation.