Related Experiment Video
Updated: Jul 14, 2026

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
Published on: October 28, 2021
Sequence variability in the FUM1 gene of Fusarium verticillioides strains
Valéria Nascimento da Silva1, Jansen de Araujo, Edison Luiz Durigon
1Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, Av Prof. Lineu Prestes, 1374, Cidade Universitária, CEP 05508-900 São Paulo, SP, Brazil. valenas@ig.com.br
Abstract:
Fumonisins are mycotoxins, produced mainly by Fusarium verticillioides, that are potentially carcinogenic to humans and toxic to animals. Synthesis of these toxins is directed by a cluster of 15 genes, among which FUM1 is the largest; it encodes a polyketide synthase. This enzyme probably catalyzes the synthesis of a polyketide that forms a large portion of the fumonisin structure. In this study, 27 strains possessing the FUM1 gene, as determined by polymerase chain reaction, were analyzed. A portion of the FUM1 gene was amplified and sequenced from 6 of 27 Brazilian strains isolated from corn and sorghum. The sequence similarity for the six F. verticillioides strains was almost 100%.
Related Concept Videos
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Dihybrid Crosses
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Monohybrid Crosses
