Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Fuel Cells01:23

Microbial Fuel Cells

Microbial fuel cells (MFCs) are bioelectrochemical devices that generate electricity by exploiting the metabolic processes of electrogenic bacteria. These systems provide a renewable energy source and serve as an innovative method for treating organic waste, such as wastewater.A typical MFC consists of two chambers: an anoxic (oxygen-free) compartment that houses the bacteria and an oxic (oxygen-rich) compartment that contains oxygen as the terminal electron acceptor. Many MFCs use proton...
Microbes and the Nitrogen Cycle01:26

Microbes and the Nitrogen Cycle

The nitrogen cycle is a complex biogeochemical process critical to maintaining the balance of nitrogenous compounds in ecosystems. This cycle involves multiple microbial-mediated transformations through which nitrogen changes oxidation states, supporting essential ecological functions and contributing to plant and microbial growth.Nitrogen Fixation and AmmonificationNitrogen fixation initiates the cycle by converting inert atmospheric nitrogen (N₂) into bioavailable ammonia (NH₃), a process...
Environmental Applications of Microorganisms01:30

Environmental Applications of Microorganisms

Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
Microbial Wastewater Treatment01:30

Microbial Wastewater Treatment

Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation. However, because inorganic electron donors...
Microbes and Methanogenesis01:26

Microbes and Methanogenesis

Methanogenesis is a critical microbial process in anaerobic ecosystems responsible for the biological production of methane, a potent greenhouse gas and valuable biofuel. This metabolic pathway is primarily facilitated by methanogenic archaea, which thrive in anoxic environments such as wetlands, sediments, and animal gastrointestinal tracts. The absence of oxygen in these habitats prevents aerobic respiration, thereby favoring alternative biochemical pathways for organic matter degradation.In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Propionate oxidation by <i>Geobacter sulfurreducens</i> is electron acceptor dependent.

Applied and environmental microbiology·2026
Same author

Growth and protein content of Cupriavidus necator on organic acids derived from fermented grey starch.

Applied microbiology and biotechnology·2026
Same author

Microbial protein-derived bioplastics from renewable substrates: pathways, challenges, and applications in a circular economy.

Environmental science and ecotechnology·2025
Same author

Mediated electron transfer in five prevalent human oral microbial species.

Bioelectrochemistry (Amsterdam, Netherlands)·2025
Same author

Mechanistic insights into fermentative pathway control during solid-state food waste acidogenesis under autogenic pressure.

Water research·2025
Same author

Low electrode potentials enhance current generation by Geobacter sulfurreducens biofilms: A high-throughput study.

Biosensors & bioelectronics·2025

Related Experiment Video

Updated: Jul 14, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

Biological denitrification in microbial fuel cells.

Peter Clauwaert1, Korneel Rabaey, Peter Aelterman

  • 1Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.

Environmental Science & Technology
|June 2, 2007
PubMed
Summary

This study introduces a novel microbial fuel cell (MFC) that simultaneously removes organic matter and nitrogen compounds from wastewater, generating power. This innovative MFC achieves complete denitrification using biological anodes and cathodes without external power or hydrogen production.

More Related Videos

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
11:58

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Published on: December 29, 2013

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Related Experiment Videos

Last Updated: Jul 14, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
11:58

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Published on: December 29, 2013

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Area of Science:

  • Environmental Science
  • Electrochemistry
  • Microbiology

Background:

  • Microbial fuel cells (MFCs) are of significant interest for simultaneous wastewater treatment and energy generation.
  • Efficient removal of both carbon and nitrogen compounds from wastewater remains a challenge in practical applications.

Purpose of the Study:

  • To develop and evaluate a MFC capable of complete denitrification using biological processes in both the anode and cathode.
  • To assess the MFC's performance in terms of nitrogen removal rate, power production, and operational parameters.

Main Methods:

  • A tubular MFC with an internal cathode and a cation exchange membrane was constructed.
  • Microorganisms in the anode oxidized acetate, supplying electrons to the cathode for denitrification.
  • Cathodic electrode potential was controlled to optimize denitrification.

Main Results:

  • The MFC achieved high nitrate removal rates (up to 0.146 kg NO(3-)-N m(-3) NCC d(-1)).
  • Significant power output was recorded (up to 8 W m(-3) NCC).
  • Effective denitrification occurred at cathodic potentials below 0 V vs. SHE, limited by microbial activity.

Conclusions:

  • This study demonstrates the first MFC with a biological anode and cathode for simultaneous organic substrate removal, power generation, and complete denitrification.
  • The developed MFC offers a sustainable solution for wastewater treatment without external power or reliance on hydrogen formation.