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Updated: Jul 14, 2026

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

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Published on: May 27, 2021

Probing phospholipid dynamics by electrospray ionisation mass spectrometry.

Anthony D Postle¹, David C Wilton, Alan N Hunt

¹School of Medicine, University of Southampton, Southampton SO17 1BJ, UK. adp@soton.ac.uk

Progress in Lipid Research

|June 2, 2007

Summary

Electrospray ionisation mass spectrometry (ESI-MS) advances lipid analysis. Combining ESI-MS with stable isotope labelling enables in vivo studies of phospholipid synthesis and turnover, aiding disease research.

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A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

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A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

Published on: August 21, 2009

Area of Science:

Biochemistry
Analytical Chemistry
Molecular Biology

Background:

Electrospray ionisation mass spectrometry (ESI-MS) has significantly improved phospholipid analysis.
Metabolic labelling with stable isotopes offers a powerful tool for studying lipid dynamics in vivo.
Lipidomics is increasingly applied in biological and clinical research.

Purpose of the Study:

To review the methodology of stable isotope incorporation for dynamic lipidomics.
To highlight studies utilizing ESI-MS and metabolic labelling for phospholipid analysis.
To summarize ESI-MS applications in characterizing phospholipids in development and disease.

Main Methods:

Utilizing electrospray ionisation mass spectrometry (ESI-MS) for phospholipid analysis.
Employing metabolic labelling with stable isotopes to trace phospholipid synthesis and turnover.
Applying dynamic lipidomic approaches to study biological systems in vivo.

Main Results:

ESI-MS coupled with stable isotope labelling allows for detailed investigation of phospholipid metabolism.
This approach facilitates the characterization of structural and signalling phospholipid species.
Numerous studies have successfully applied these techniques in various biological and clinical contexts.

Conclusions:

The combination of ESI-MS and stable isotope labelling is a powerful strategy for dynamic lipidomics.
This methodology provides crucial insights into phospholipid synthesis, turnover, and function.
These advancements are instrumental in understanding phospholipid roles in development and disease.