Related Experiment Video
Updated: Jul 14, 2026

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
Published on: January 7, 2015
Effect of interleukin-13 or tumor necrosis factor-alpha on eosinophil adhesion to endothelial cells under
Hiroshi Tachimoto1, Motohiro Ebisawa
1Department of Allergy, Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Japan. tachimoto@sagamihara-hosp.gr.jp
Rationale:
We examined the mechanisms used by eosinophils to accumulate on IL-13- or TNF-alpha-stimulated human umbilical vein endothelial cells (HUVECs) under flow conditions.
Methods:
HUVECs were treated for 1, 3, 6, 18 and 24 h with IL-13 or TNF-alpha (1-100 ng/ml). Human eosinophils were infused at physiologic flow rates (0.5 dyn/cm(2)) for 10 min, and attached eosinophils were counted.
Results:
Under these flow conditions, eosinophils accumulated efficiently on IL-13-stimulated (109 +/- 18 cells/field) or TNF-alpha-stimulated (96 +/- 27 cells/field) HUVECs in a concentration-dependent manner. Eosinophil accumulation on IL-13-activated HUVECs was first observed at 3 h and reached a maximum at 24 h. On the other hand, the levels of eosinophils accumulating on TNF-alpha-activated HUVECs were the same at all time points (1, 3, 6, 18 and 24 h). Anti-alpha4 integrin mAb inhibited eosinophil accumulation on both IL-13- and TNF-alpha-activated HUVECs.
Conclusions:
Eosinophil accumulation on HUVECs under physiologic flow conditions is differentially regulated by IL-13 and TNF-alpha.
Related Concept Videos
Intracellular Signaling Affects Focal Adhesions
Some...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

