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Updated: Jul 14, 2026
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Increased serum levels of nitrotyrosine, a marker for peroxynitrite production, in systemic sclerosis
1Department of Dermatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Objective:
To determine serum levels of nitrotyrosine (NT), an end product of peroxynitrite (ONOO-), and its clinical association in patients with systemic sclerosis (SSc).
Methods:
Serum NT levels from 25 patients with limited cutaneous SSc (lSSc) and 34 patients with diffuse cutaneous SSc (dSSc) were examined by enzyme-linked immunosorbent assay.
Results:
Serum NT levels were elevated in SSc patients compared with normal controls (n = 27), the levels being similar between lSSc and dSSc patients (P < 0.001). SSc patients with elevated NT had higher serum levels of anti- agalactosyl IgG Ab, IgG and IgA than those with normal NT levels (P < 0.05). NT levels correlated inversely with the percentage diffusion capacity for carbon monoxide (DLco) (P < 0.02, r = -0.414, n = 47).
Conclusion:
These results suggest that ONOO- may play an important role in the clinical manifestations of SSc, especially vascular damage to the lungs, and that ONOO- may be related to immunological abnormalities in SSc.
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