Ultrastructural and immunohistochemical characteristics of developing human pituitary gland

The development and differentiation of the human pituitary gland and its relationship to other structures of the head were analysed in nine human embryos and fetuses aged 5-10 weeks old using morphological and immunohistochemical methods. In the 5th developmental week, the primordium of Rathke's pouch was closely associated with the cranial tip of the notochord, head mesenchyme and diencephalon. Cells of the Rathke's pouch displayed typical epithelial features that transformed into gland-like structures during development. Numerous Ki-67 positive cells characterised the Rathke's pouch, the diencephalon (neurohypophysis) and the associated mesenchyme. The highest proliferation rate was noticed in the earliest developmental stage, while it significantly decreased in the 7th week of development. The first intermediate filaments to appear in the Rathke's pouch showed cytokeratin 8 immunolabelling which decreased with advanced maturation. The diencephalon and infundibulum displayed parallel immunolabelling of vimentin, glial fibrillary acidic protein (GFAP) and neurofilament protein (NF), while the surrounding mesenchyme showed only vimentin labelling. Changes in the labelling of Ki-67 proliferation marker and intermediate filament proteins in the developing human pituitary gland coincided with separation of the Rathke's pouch from the pharyngeal epithelium and subsequent differentiation of different parts of the gland.