Related Experiment Video
Updated: Jul 14, 2026
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile
Pawel L Urban1, David M Goodall, Edmund T Bergström
1Department of Chemistry, University of York, Heslington, York YO10 5DD, UK.
Abstract:
An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50 mM borate, pH 8.8). Results are presented for parallel capillary electrophoresis with a novel miniature UV area detector, with an active pixel sensor imaging an array of two or six parallel capillaries connected via a manifold to a single output capillary in a commercial CE instrument, allowing conversions with five different yeast alcohol dehydrogenase concentrations to be quantified in a single experiment.
