Preparation of biomolecule gel matrices for electron microscopy

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Preparation of Complaint Matrices for Quantifying Cellular Contraction
11:38

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Published on: December 14, 2010

Sonal Padalkar1, Jianxiu Zhao, Kate Stuart

  • 1School of Materials Engineering, Purdue University, West Lafayette, IN 47907-2044, USA.

Ultramicroscopy
|June 5, 2007
PubMed

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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...