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Updated: Jul 14, 2026

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System
Published on: December 21, 2019
[Specific small interfering RNAs inhibit replication of hepatitis B virus]
Jing Jiao1, Hong Cao, Mei-juan Zhou
1Department of Radiation Medicine, Southern Medical University; Guangzhou 510515, China. jiaojing_pop@163.com
Objective:
To obtain specific small interfering RNAs (siRNA) for hepatitis B virus (HBV) and evaluate their interfering effect.
Methods:
Three siRNAs were transfected into HepG2.2.15 cells, and the amount of HBV mRNA in the cell culture medium was quantified with real-time fluorescence quantitative RT-PCR. HBsAg in the culture media was assayed with Western blotting at different time points after transfection.
Results:
The cells transfected with specific siRNAs showed decreased levels of HBV mRNA and HBsAg (P<0.05), but those with nonspecific siRNA transfection as the negative control did not show such changes (P>0.05).
Conclusion:
Specific siRNA can significantly inhibit protein expression and mRNA synthesis of HBV in HepG2.2.15 cells in vitro.
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