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Updated: Jul 14, 2026
A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
Published on: May 17, 2024
[Cloning of human PRL-3 gene and construction of its prokaryotic expression vector]
Jun Zhou1, Jian-ming Li, Yu-hong Liu
1Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Objective:
To obtain the entire coding sequence of human PRL-3 gene and construct its prokaryotic expression vector.
Methods:
With total RNA extracted from the human colorectal carcinoma cell line SW480 as the template, PRL-3 gene was amplified by RT-PCR with primers designed according to the published sequence of GenBank, and the product was inserted into pGEM-T Easy vector. The recombinant plasmid pGEM-T-PRL-3 was identified by restriction endonuclease analysis and DNA sequencing. After digestion with restriction endonuclease, PRL-3 gene was cloned into the multicloning sites of the prokaryotic expression vector pGEX-4T-1.
Results And Conclusion:
The entire coding region of human PRL-3 gene was cloned, and the recombinant pGEX-4T-1-PRL-3 vector was successfully constructed and expressed, which may provide the basis for further study of the relationship between human colorectal carcinoma and PRL-3 gene.
