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An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
[Construction of eukaryotic expression vector for VEGF165 gene and its expression in rat bladder smooth muscle cells]
Mao-hu Lin1, Shan Zhao, Rui Miao
1Postgraduate School, Fourth Military Medical University, Xi'an 710032, China.
Objective:
To construct an eukaryotic expression vector for vascular endothelial growth factor (VEGF) 165 gene and obtain VEGF expression in rat bladder smooth muscle cells.
Methods:
VEGF165 cDNA was cloned into the eukaryotic expression vector pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/VEGF165 was transfected into the rat bladder smooth muscle cells by electroporation. VEGF expression in the cells was determined by RT-PCR and immunofluoresence assay, and the biological activity of VEGF in the supernant of the transinfected cell culture was tested by MTT assay.
Results And Conclusion:
VEGF expression was obtained in the transinfected cells, and the supernant of the transinfected cell cultures stimulated the proliferation of the endothelial cells.
