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Related Experiment Videos

Collagen-induced arthritis.

David D Brand1, Kary A Latham, Edward F Rosloniec

  • 1Research Service, Veterans Affairs Medical Center, Memphis, Tennessee 38104, USA. dbrand@utmem.edu

Nature Protocols
|June 5, 2007
PubMed
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This protocol details the collagen-induced arthritis (CIA) mouse model for rheumatoid arthritis research. It enables reproducible induction and evaluation of CIA in susceptible mice, aiding autoimmune disease studies.

Area of Science:

  • Immunology
  • Rheumatology
  • Animal Models

Background:

  • Rheumatoid arthritis (RA) is a significant autoimmune disease.
  • The collagen-induced arthritis (CIA) mouse model is widely used to study RA pathogenesis.
  • Standardized protocols are crucial for reproducible results in CIA studies.

Purpose of the Study:

  • To provide a detailed protocol for inducing and evaluating collagen-induced arthritis (CIA) in mice.
  • To ensure reproducible induction of CIA in genetically susceptible mouse strains.
  • To guide researchers in the preparation of reagents and immunization techniques.

Main Methods:

  • Acquisition, handling, and preparation of type II collagen (CII).
  • Selection of appropriate mouse strains for CIA induction.

Related Experiment Videos

  • Detailed immunization techniques using CII and complete Freund's adjuvant.
  • Evaluation of arthritis incidence and severity.

    • Main Results:

    • The protocol allows for reproducible induction of CIA in susceptible mouse strains.
    • Arthritis onset typically occurs 21-28 days post-immunization.
    • The method facilitates critical evaluation of disease pathology.

    Conclusions:

    • This protocol enables reliable induction and assessment of collagen-induced arthritis.
    • It serves as a valuable resource for researchers studying autoimmune arthritis and RA.
    • The standardized approach supports consistent and comparable experimental outcomes.