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Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy
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Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy

Benjamin J Zeskind1, Caroline D Jordan, Winston Timp

  • 1WI-MIT BioImaging Center, 500 Technology Square NE47-287, Cambridge, Massachusetts 02142, USA. bzeskind@alum.mit.edu

Nature Methods
|June 5, 2007
PubMed

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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...