[Gene therapy of bone morphogenetic protein-2 for periodontal tissue regeneration in vivo]
Qin-feng Sun1, Xue-mei Zhu, Pi-shan Yang
1Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, Shandong Province, China.
Purpose:
To evaluate the effect of pIRES-rhBMP-2 gene on the regeneration of periodontal bone defects of beagle dogs in vivo.
Methods:
Following surgery to create a U-shaped periodontal osseous defect on the buccal aspect of 6 mandibular premolar (P2,P3,P4) mesial roots, 6 adult beagle dogs were divided into three groups, pIRES-rhBMP-2 group, rhBMP-2 group and phosphate-buffered saline (PBS) group respectively. The animals were sacrificed after 4 or 8 weeks and the mandible taken for histological examination. Histometric measurements were performed with Image-Pro- Express system. The height of new bone and cementum and the formation of new connective tissue were analyzed and compared. The results were analyzed by N-K test using SAS6.12 software package.
Results:
After 8 weeks, a complete osseous healing occurred and dense new periodontal ligament fibers rich in blood vessels were observed in the pIRES-rhBMP-2 group and the rhBMP-2 group, whereas fewer new bone occurred and sparse collagen fibers aligned irregularly were observed in the blank control group. The height of new bone and cementum and the formation of new connective tissue were significantly greater in the two experimental group than in the blank control group (P<0.05,P<0.01), whereas there was no significant difference in the two experimental groups (P>0.05).
Conclusion:
pIRES-rhBMP-2 can accelerate the regeneration of dogs' periodontal bone defects.
