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Related Experiment Video

Updated: Jul 14, 2026

Experimental Column Setup for Studying Anaerobic Biogeochemical Interactions Between Iron (Oxy)Hydroxides, Trace Elements, and Bacteria

Experimental Column Setup for Studying Anaerobic Biogeochemical Interactions Between Iron (Oxy)Hydroxides, Trace Elements, and Bacteria

Published on: December 19, 2017

Sulfide increases labile iron pool in RD4 cells.

Jonas Hälldin¹, Tiit Land

¹Department of Neurochemistry, Stockholm University, Stockholm, Sweden. jonas@neurochem.su.se

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine

|June 5, 2007

Summary

Sulfide treatment increases labile iron pool (LIP) in muscle cells, suggesting a link between sulfur metabolism and iron regulation. This study provides in vivo evidence for this connection.

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Area of Science:

Cellular metabolism
Biochemistry
Physiology

Background:

A potential link between sulfur and iron metabolism exists, as sulfide can release iron from ferritin in vitro.
However, in vivo and cellular evidence supporting this connection remains limited.

Purpose of the Study:

To investigate the relationship between sulfur metabolism and intracellular iron regulation in mammalian cells.
To determine the effect of sulfide on iron bioavailability in a cellular model.

Main Methods:

Human RD4 skeletal muscle cells were treated with sodium sulfide.
Measurements included intracellular sulfide concentration, labile iron pool (LIP) levels, L-ferritin protein, and iron regulatory protein 2 (IRP2) levels.

Main Results:

Sulfide treatment led to a 100% increase in LIP levels within 1-2 hours.
Intracellular sulfide concentrations rose by 60%, correlating with increased LIP.
Increased iron bioavailability was indicated by a 100% rise in L-ferritin and a 60% decrease in IRP2.

Conclusions:

The findings suggest a direct linkage between sulfur metabolism and intracellular iron regulation in mammalian cells.
Sulfide plays a role in modulating iron availability within the cellular environment.