[Polymorphic analysis of intron 2 and 3 of growth hormone gene in duck]

The single nucleotide polymorphism (SNP) of growth hormone gene was investigated in various breeds of duck, including Beijing ducks, Xihu mallards, Jinding ducks, Shan Partridge ducks, Jingjiang ducks and Shaoxing ducks. The primers for intron 2 and 3 in GH gene were designed based on the database of duck genomic sequence and the SNPs were detected by PCR-SSCP method. Eight SNPs were found among individuals within a breed, which were 2593(C-T), 2770(G-A), 2813(T-A), 2829(C-A), 2894(C-T), 2896(T-C), and 3100(C-G) in intron 2 and 3270(A-G) in intron 3. The analytic results showed that the frequencies of genotypes in different breeds were significantly different. Based on these SNPs, Beijing ducks and Shaoxing ducks represented their own unique conservativeness, indicating that these SNPs may have relationship with some productive traits of duck.