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Adhesion of Helicobacter pylori to human gastric mucosal biopsy specimens cultivated in vitro
K Rosberg1, T Berglindh, S Gustavsson
1Dept. of Pharmaceutical Pharmacology, Uppsala University, Sweden.
Abstract:
The therapeutic advances in Helicobacter pylori infection is hampered by the lack of suitable animal model systems. We have previously reported on successful adherence of H. pylori to pig gastric mucosa cultured in vitro. The aim of this study was to verify the technique in human biopsy specimens cultured in vitro. Mucosal samples were taken from H. pylori-negative and H. pylori-positive patients undergoing gastric surgery. The non-infected tissue was infected with H. pylori in vitro, and the infected tissue was put into culture immediately. Total number and those H. pylori firmly attached were checked throughout a 72-h culture. Viability of cultured human gastric mucosa was good and unaffected by the presence of H. pylori. The amount of bacteria adhering, increased with time from 0.01% to 2-4% after 72 h in culture. In vivo-infected specimens initially had a low number of firmly attached H. pylori, but total H. pylori increased with time in culture. It is concluded that human gastric biopsy specimens show good viability for 72 h and that viability and cell division of H. pylori were maintained in both in vivo and in vitro H. pylori-infected tissue. In both cases the total number of viable bacteria attached to the specimens increased with incubation time.
Insights
Human gastric biopsy cultures support Helicobacter pylori growth and attachment for 72 hours. This model offers a viable alternative for studying H. pylori infection and developing new therapies.
Area of Science:
- Gastroenterology
- Microbiology
- Infectious Diseases
Background:
- Therapeutic advancements for Helicobacter pylori infections are limited by the absence of suitable animal models.
- Previous research demonstrated H. pylori adherence to cultured pig gastric mucosa.
Purpose of the Study:
- To evaluate the efficacy of using human gastric biopsy specimens for in vitro culture of H. pylori.
- To assess the viability and bacterial attachment over time in cultured human gastric tissue.
Main Methods:
- Human gastric mucosal samples were obtained from H. pylori-negative and H. pylori-positive patients.
- Non-infected tissue was cultured and exposed to H. pylori in vitro; infected tissue was immediately cultured.
- Bacterial adherence and viability were monitored over a 72-hour incubation period.
Main Results:
- Cultured human gastric mucosa maintained good viability for 72 hours, irrespective of H. pylori presence.
- The percentage of adhering H. pylori increased from 0.01% to 2-4% over 72 hours in vitro.
- Both in vivo and in vitro infected tissues showed increased total viable H. pylori attachment over time.
Conclusions:
- Human gastric biopsy specimens provide a viable model for studying H. pylori for up to 72 hours.
- The model supports both H. pylori viability and cell division, mimicking infection conditions.
- This in vitro human tissue model is valuable for research into H. pylori pathogenesis and treatment strategies.