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Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis
Published on: March 11, 2017
Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose
Pan-Kyeom Kim1, Mi-Ryung Kim, Hyun-Jung Kim
1School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Proteomics
|June 6, 2007
Abstract:
To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.
