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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Updated: Jul 14, 2026

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

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Published on: October 28, 2022

A better technique for taking Mohs sections involving cartilage.

Ravi S Krishnan¹, Heidi B Donnelly

¹Dayton Skin Surgery Center, Dayton, Ohio 45429, USA.

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [Et Al.]

|June 7, 2007

Summary

A modified Mohs surgery technique improves cartilage margin examination by creating flatter bevels. This allows for more thorough assessment and successful tumor removal, especially in complex cases.

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Last Updated: Jul 14, 2026

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

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Published on: October 28, 2022

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The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

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Published on: September 5, 2018

Area of Science:

Dermatology
Surgical Pathology

Background:

Conventional Mohs surgery struggles to flatten inelastic cartilage margins for complete examination.
This limitation can hinder accurate assessment of surgical margins in cartilaginous specimens.

Purpose of the Study:

To present a modified Mohs technique for excising skin and cartilage specimens.
The goal is to facilitate complete flattening of the entire specimen margin for thorough examination.

Main Methods:

The modified technique involves excising an additional 1-2 mm of skin around the cartilage.
Creating flatter bevels at 20-30 degrees on the specimen edges is proposed.

Main Results:

The modified approach enables easy flattening of skin edges, cartilage edges, and the deep cartilage surface onto a single plane.
This facilitates a comprehensive examination of all surgical margins.

Conclusions:

The technique enhances margin assessment, leading to more successful tumor extirpation.
Potential limitation: slightly increased tissue excision may impact cosmetic outcomes in sensitive areas.