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Updated: Jul 14, 2026

Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
Dynamic resolution of functionally related gene sets in response to acute heat stress
Joseph D Szustakowski1, Penelope A Kosinski, Christine A Marrese
1Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA. joseph.szustakowski@novartis.com <joseph.szustakowski@novartis.com>
Background:
Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways.
Results:
Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours.
Conclusion:
In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.
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