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Related Experiment Video

Updated: Jul 14, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Intermediate vancomycin susceptibility in a community-associated MRSA clone.

Christopher J Graber¹, Margaret K Wong, Heather A Carleton

¹Division of Infectious Diseases, University of California, San Francisco General Hospital, 94110, USA. christopher.graber@ucsf.edu

Emerging Infectious Diseases

|June 8, 2007

Summary

A USA300 community-associated methicillin-resistant Staphylococcus aureus (MRSA) strain showed treatment failure due to intermediate vancomycin susceptibility. This MRSA strain also had reduced daptomycin susceptibility, complicating osteomyelitis treatment.

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Last Updated: Jul 14, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Area of Science:

Infectious Diseases
Microbiology
Clinical Medicine

Background:

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) USA300 is a prevalent pathogen.
Osteomyelitis requires effective antibiotic treatment.
Catheter-associated septic thrombophlebitis can lead to serious infections.

Observation:

A 56-year-old male patient with lumbar osteomyelitis experienced treatment failure.
The patient received a 6-week course of vancomycin for catheter-associated septic thrombophlebitis.
The causative MRSA strain exhibited intermediate vancomycin susceptibility and reduced daptomycin susceptibility.

Findings:

The MRSA strain demonstrated reduced susceptibility to vancomycin and daptomycin.
Treatment failure occurred despite vancomycin therapy.
The bone infection was caused by this specific MRSA strain.

Implications:

Emerging antibiotic resistance in MRSA poses a significant clinical challenge.
Vancomycin and daptomycin may be less effective against certain MRSA strains.
Alternative treatment strategies are needed for osteomyelitis caused by resistant MRSA.