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[Determination of aisaticoside in Centella asiatica extract by HPLC-ELSD]
Quan-Lin Yu1, Wen-Yuan Gao, Hai-Xia Chen
1College of Pharmaceutical Sciences and Biotechnology, Tianjin University, Tianjin 300072, China.
Objective:
A HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract.
Method:
The column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1).
Result:
The linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%).
Conclusion:
The method is reliable, simple, precise and could be used for the quality control of C. asiatica extract.
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